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Aemtek, Inc. Storefront
Aemtek is an AIHA accredited environmental microbiology laboratory located in the Bay Area, California. We specialize in the detection, analysis, and research of fungi and bacteria. We are committed to excellence in quality, service, and technology.
We use morphological, physiological, biochemical, and molecular methods for analysis. We accept various sample matrices including air, bulk, culture, swab, tape, dust, water samples. In addition, we conduct contract research in a wide range of subjects in applied and environmental microbiology and mycology (see About Aemtek). Our analysts received vigorous training in accredited universities and have many years of microbiological and mycological experiences, as detailed in Experiences and Qualifications. We are dedicated to providing the most accurate analysis, while being responsive to your time-sensitive analytical needs. We thrive on ensuring the highest standards of quality and excellence while providing unparalleled customer service. As a result, our clients believe that we are the best mycology lab in the country (see Testimonials). Our analytical protocols are either standard methods or developed in house based on scientific merits. Aemtek is accredited by the American Industrial Hygiene Association (AIHA) Environmental Microbiology Laboratory Accreditation Program (Lab No.: 167620, see Laboratory Accreditations) As a value adding service to our clients, we publish a monthly newsletter, Micro Examiner . Each issue contains a technical article about an applied and environmental microbiology issue, a profile of a microorganism, and useful news and updates. You are welcome to subscribe to the newsletter. We invite qualified and dedicated microbiologists and mycologists to join us. Our goal is to get together a collection of brilliant minds, hearts, and talents to make Aemtek a masterpiece in technological entrepreneurship. Call Aemtek at (510) 979-1979 to ensure that your microbial investigations are done right the first time, every time, and on time!
Sign Up and Stay Informed Sign up for Aemtek’s monthly newsletter the MicroExaminer. Each month features industry related news, updates, an in-depth article, and a microbial profile. Just enter your email in the Sign up box: Sign up here Offer Terms & RestrictionsEmail address is required to sign up. Thank you! | Free sampling supplies (swab, tape, & media)Free sampling supplies (swab, tape, & media) with analytical order | Free analysis of Air-O-Cell samples For a limited time, Aemtek offers free analysis of up to 4 Air-O-Cell samples for new customers. Offer Details | Free In-bound shippingFree inbound sample shipping for orders over $200. | |
Aemtek Hires New StaffAemtek is pleased to announce the addition of two new members to our environmental laboratory team, Jerry Oliveras and Danielle Rotondo. Jerry will be opening and directing Aemtek's new chemistry lab which will be offering chemical testing and Danielle will assist our valued clients and play an integral part in helping to further build and promote the capabilities of Aemtek. Before joining Aemtek, Jerry was the Laboratory Director & President of a well-respected commercial food laboratory located in San Francisco, CA. He has over 20 years of professional experience. He has worked in several environmental, food and agricultural testing laboratories and has experience in the fields of food and water microbiology, analytical chemistry, laboratory management, regulatory compliance, and chemical research. He is a recognized expert in food chemistry, microbiology and technology with wide experience in troubleshooting industry problems. Danielle was previously the Public Relations Specialist for Catapult Direct Marketing and Target Profiling. Prior to that, Danielle practiced Public Relations for various consultants in the San Francisco Bay Area, while pursuing her Bachelors of Science in Public Relations and minor in Psychology at San Jose State University. To learn more about their qualifications and experiences, go to the The Aemtek Team page. Aemtek Launches New WebsiteWe're pleased to present the newly redesigned Aemtek website. The new site has user-friendly features and a new navigation menu. The Resource section is enriched with an updated Microbial Database, Sampling Guides, Aemtek Technical Articles, RSS Feeds, Regulations & Guidelines, and Industry Links. Industry related events will be announced on a new event calender and a Meet the Aemtek Team section is revised under the Company section where you can learn about the experiences and qualifications of Aemtek's staff. As always, we welcome your comments and feedback. Visit us: http://www.aemtek.com Aemtek provides accurate, fast and reliable laboratory services and our commitment to scientific excellence, quality service and advanced technology assures our clients that their projects are done right the first time, every time, and on time. For more information, contact Danielle Rotondo at (510) 979-7979. Aemtek Is ELAP AccreditedThe State of California Department of Health Services Environmental Laboratory Accreditation Program (ELAP) has granted Aemtek, Inc. Environmental Laboratory Certification (Certification No. 2607). ELAP Accredited Fields of Testing include microbiology of drinking water, recreational water and wastewater. Common Indoor Allergens Allergens from dust mites, cat, dog, cockroach, rodents, and mold pose one of the most common serious health threats in indoor environments. The allergens produced by these organisms, can cause allergic symptoms by becoming aerosolized and entering the human airways or by direct contact. Respiratory symptoms typically include wheezing, coughing, sinusitis, chronic or perennial rhinitis, and allergic asthma. When these allergens come in contact with the skin, it may cause, dermatitis (eczema), hives and itching; or in the eyes, it may cause itching, burning, swelling, and watering. Some allergens such as dust mites, cockroach and cat also have been shown to exacerbate symptoms or trigger asthma. In addition to their allergenic potential, cockroaches and rodents pose serious threats as disease vectors. - Dust Mite Allergens
Dust mite allergens are considered one of the most potent allergens found in indoor environments. The two most commonly found dust mite species in the U.S are Dermatophagoides farinae and D. pteronyssinus. Allergens of these species are known as Der f1 and Der p1 respectively. Dust mite allergens can easily become airborne when dust is disturbed. It has been documented that exposure in early childhood to house-dust mite allergens is an important determinant of the subsequent development of asthma. - Dog and Cat Allergens
Of the domesticated animals found in urban areas, cats are one of the most allergenic. Studies have shown that sufficient cat allergen can be carried on owners clothing and it can cause severe allergic reactions in co-workers that do not own cats. Dogs are far less allergenic than cats. Allergic reactions to both cats and dogs are caused by proteins found mainly in the animal's saliva, urine, and to a lesser extent in their dander. Fel d 1 and Can f 1 are the most common allergens from cat and dog, respectively. They are small-molecular-weight proteins that are able to circulate on the air currents throughout homes. In additions, these allergens are also sticky and become easily attached to clothes, walls, and furniture, making it virtually impossible for any room to be completely allergen-free. As a result, several reports have shown that dog and cat allergens are present in more than 99% of the homes in the US regardless of the presence or absence of these pets at the time of testing. - Cockroach Allergens
Allergy reactions caused by cockroaches are produced from exposure to their saliva, fecal material, eggs, secretions, and cast off skin. Cockroach allergens are similar to those of dust mites in that they occur in house dust and bedding. The most common cockroach allergens found in the US are from the German cockroach (Blatella germanica) the Bla g 1 and Bla g 2 to which analytical tests are available. The symptoms of cockroach allergy do not differ from other types of inhalant allergies. - Rodent Allergens
Rodent allergens include rat and mice allergens. These allergens are especially important in laboratories where these animals are handled for research and in establishments and in homes where mice and rats are kept as pets as well as in inner-city houses or buildings. Allergic reactions are most common in people handling the animal's bedding and cage-washing. Mouse allergens have been found in over 80% of the US households in the inner-city areas. These allergies are mainly caused by the rodent's saliva, urine, and dander. In addition to the allergenic potential, rodents can pose a serious threat to humans by carrying diseases such as Hantavirus. Exposure to such diseases is generally rare but when it does occur, can cause severe health problems or even death. - Mold Allergens
Molds are certain kind of fungi that grow by producing mycelia which many times looks fuzzy or velvety. Molds produce tiny spores to reproduce that can move through indoor and outdoor air continually. When mold spores land on a damp spot indoors, they may begin growing and digesting whatever they are growing on such as wood, paper, carpet, drywall, or foods. When excessive moisture or water accumulates indoors, mold growth will often occur, particularly if the moisture problem remains undiscovered or un-addressed. References Bioaerosols: Assessment and Control, Janet Macher, Sc.D., M.P.H., Editor. 1999. ACGIH, 1330 Kemper Meadow Drive, Cincinnati, OH 45240-1634. Phipatanakul W. 2002. Rodent Allergens. Curr. Allergy Asthma Sep.2 (5) 412-6 (Abs) Stuart Greenberg. 2003. Cockroach Allergen Reduction Using Precision-Targeted IPM and the Lead Dust Cleaning Protocol. Final Report. Cooperative Agreement #OHLHH0069-99. Environmental Health Watch, Cleveland Ohio. Websites www.aaaai.org/media/news_releases/ http://www8.utsouthwestern.edu/utsw/cda/dept37389/files/210524.html http://lancaster.unl.edu/enviro/pest/factsheets/ www.aeclp.org/hhe/hhe_rodents.htm www.epa.gov/mold/moldresources.html http://www.acgih.org/ http://www.aiha.org/ http://www.allergies.about.com/ http://www.allergyweb.com/ http://www.cal-iaq.org/ http://www.calepa.ca.gov/ Microorganisms and Foodborne DiseasesThe US Centers for Disease Control and Prevention estimates foodborne pathogens cause 76 million illnesses, 325,000 hospitalizations, and 5,000 deaths each year in America. The USDA Economic Research Service estimates the cost from foodborne pathogens is in excess of $6.9 billion; including medical costs, missed work time and the costs of premature deaths. With the US food and agricultural industries totaling in excess of $306 billion in revenues annually, the amount spent on monitoring safety, quality and preventing foodborne illness is staggering. Foodborne diseases are caused by ingesting bacteria, fungi, parasites, viruses or their toxins through contaminated food or drinking water. More than 200 known diseases are transmitted through food, and more than half of all recognized foodborne disease outbreaks have unknown causes, indicating the real number of disease-causing agents is likely much larger than 200. The symptoms of foodborne illnesses range from mild gastroenteritis to life-threatening neurological, hepatic, and renal syndromes. A pathogen's ability to cause illness can be very different from the severity of the illness it causes. Some pathogens cause a great number of illnesses but the severity is low. Others cause few illnesses, but those illnesses are very severe or even fatal. In addition to disease caused by direct infection, some foodborne diseases are caused by the presence of a toxin in the food that was produced by a microbe in the food. Staphylococcus aureus can grow in food and produce a toxin that causes severe vomiting. The rare yet deadly disease botulism occurs when Clostridium botulinum grows in food and produces a powerful paralytic toxin. These toxins can produce illness even if the microbes that produced them are no longer there, commonly having been killed by some heat treatment as with canned foods. Foodborne pathogens can cause actual infections in the bloodstream or tissues, in the gut or simply pass thru leaving toxins behind which cause the symptoms of foodborne illness. Each agent has a specific typical progression of illness and symptoms. Since symptoms commonly include vomiting and diarrhea, a laboratory test is required to identify the causative agent. The major culprits in bacterial foodborne illness are Salmonella, Listeria, Campylobacter, Clostridium, Shigella, E. coli 0157:H7 and Staphylococcus. Listeria and E. coli 0157:H7 are also considered emerging pathogens since there role in food illness have only become well recognized in the past 20 years. The first major outbreak of E. coli 0157:H7 was reported in 1982 and an early major outbreak with Listeria occurring in 1985 from California produced soft cheese. Recent outbreaks in fresh produce of E. coli 0157:H7 from a number of sources demonstrate that this previously animal product (beef) associated pathogen is a threat throughout the food system. Another important and common emerging pathogen is not a bacterium at all, but a virus. The Calicivirus or Norwalk-like virus is an extremely common causative agent but is rarely diagnosed due to a lack of a widely available laboratory test for it. It causes an acute gastrointestinal illness, usually with more vomiting than diarrhea, which resolves within two days. Unlike many foodborne pathogens that have animal reservoirs, it is believed that Norwalk-like viruses spread primarily from one infected person to another. The frequency of outbreaks caused by these emerging organisms has been increasing worldwide, including in the western developed countries. New foodborne disease threats occur for a number of reasons. These include increase in international travel and trade, microbial adaptation, changes in the food production system, and human demographics or behavior pattern shifts.
An Overview of 'Lumber Yard Mold'By Florence Wu, PhD. Principal Mycologist, Aemtek, Inc. “Lumber yard mold” is a term often encountered in indoor air quality surveys and building construction related projects. It is often used by many as a term to indicate certain sap stain fungi, such as species of Ceratocystis or Ophiostoma, while others believe that it encompasses a wide variety of filamentous fungi that are capable of growing on wood. In reality, green wood stored in lumber yard or exposed during construction are a potential habitat for many saprotrophic fungi. If the conditions are conducive to fungal growth, it is more likely that a variety of fungi are growing on lumber. As an example, a related study showed that Douglas fir sapwood was colonized by more than 45 species of fungi within six weeks of sawing. Wood inhabiting fungi can be roughly grouped into surface, sap stain and decay fungi, based on their ability to penetrate the wood tissue. This article attempts to provide an overview of the fungi that can grow on wood either in a lumber yard or in a building construction site. Surface mold
Some mold species, including species of Alternaria, Aspergillus, Cladosporium, Graphium, Penicillium, Phoma, Trichoderma etc, can grow on the surface of freshly cut wood. These fungi utilize the simple carbohydrates (e.g., sugar, starch) in the wood as nutrient source. The surface mold causes discoloration of the wood surface by their pigmented spores and/or mycelia, but they generally do not damage the wood structure. Sap stain fungi Sap stain fungi affect the sapwood of lumber. Common sap stain fungi include species of Ceratocystis, Ophiostoma, and Ceratosystiopsis, black yeasts, such as Hormonema dematioides, Aureobasidium pullulans, Rhinocladiella atrovirens, and Phialophora spp., and dark mold species, such as Alternaria alternata, Cladosporium sphaerospermum and C. cladosporioides. Sap stain fungi utilize sugars, starch, protein and fats stored in the sap wood tissue as nutrients. They do not significantly decompose the wood cell wall components such as cellulose, semi-cellulose, and lignin, but may reduce the wood strength and increase wood permeability making the wood vulnerable to further microbial attack. Sap stain fungi cause discoloration of wood tissue by their darkly pigmented mycelia, spores, and melanin produced by the fungi. Some species of Ceratocystis and Ophiostoma are pathogenic which means they can attack living trees, while others are saprophytic and colonize the wood after it is cut and exposed to favorable conditions for fungal growth. Ceratocystis and Ophiostoma are often among the first fungi to colonize freshly cut wood. In nature, the way by which the fungi get into the sap wood is somewhat elaborate and interesting. The spores of these fungi can adhere to the bodies of wood-dwelling insects, especially bark beetles, and be carried into the wood tissue through the insects’ activity. Species of Ceratocystis and Ophiostoma are common on wood frames in buildings. Based on our experience, the Ceratocystis/Ophiostoma colonies found in indoor air quality surveys are often deteriorated, desiccated, and spore-less. This may imply an old existence rather than a new growth. These two genera are not differentiated in laboratory report because identification of species require culturable sample. Wood decay fungi
Often neglected in indoor air quality survey, the wood decay fungi pose real damage to building structure. The wood decay fungi found in buildings are mostly basidiomycetes that become adapted to living on the wood products used for construction. The most infamous wood rotting fungi include Antrodia radiculosa, A. vaillantii, Leucogyrophana pinastri, Meruliporia incrassata, Oligoporus placenta, Serpula lacrymans and Tyromyces palustris, etc. Wood rotting fungi attack both the sapwood and heartwood and can penetrate into the wood tissue destroying the wood structure. Wood decay can be classified into white rot and brown rot, based on the wood residue left by the decay fungi. “Dry rot” is a form of brown rot caused by waterconducting decay fungi, while “soft rot” refers to decay caused by Ascomycete fungi such as Chaetomium and mold such as Stachybotrys. Indoor air quality issue It is generally believed that fungi cannot grow on a wood when its water content is below 19%. Properly treated and dried out wood products usually do not support fungal growth. From building construction points of view, prohibiting or reducing fungal growth on lumber requires effective moisture control throughout the entire process of utilizing wood as building material, including manufacturing, lumber mill operations, storage, shipping, and construction process. For building management, preventing water from penetrating into a wood material is an effective way to avoid its decay. Surface molds that are dead and enclosed in building structures that inhibit their dispersal into the indoor air are probably not a health risk. Similarly lumber yard mold such as Ceratocystis and Ophiostoma on dry wood frames between walls or enclosed in building structures do not necessarily pose any mold contamination concern or health risk. However, chronic and prolonged wetting of the wood structures of a building often cause its decay, which should be addressed due to its structural damage. When damp conditions allow various saprotrophic fungi to flourish in an indoor environment, the situation becomes a familiar indoor air quality problem, not just a lumber yard mold problem. Comments? Send an e-mail to florencewu@aemtek.com About Aemtek Aemtek is an environmental microbiology laboratory providing accurate, fast, and reliable data. We specialize in detection, analysis, and research of fungi and bacteria using direct examination, culture method, PCR, and DNA sequencing. We are committed to excellence in quality, service, and technology. If you would like to subscribe to this newsletter, please sign up online at our website at http://www.aemtek.com and fill out your information, follow the instructions in the confirmation email, and you will start to receive every issue in your inbox each month. 46309 Warm Springs Blvd., Fremont, CA 94539; Phone: 510-979-1979; Fax: 510-668-1980; info@aemtek.com; www.aemtek.com Microorganisms and Foodborne Diseases By Jerry Oliveras, PhD. Chemistry Director, Aemtek, Inc. The US Centers for Disease Control and Prevention estimates foodborne pathogens cause 76 million illnesses, 325,000 hospitalizations, and 5,000 deaths each year in America. The USDA Economic Research Service estimates the cost from foodborne pathogens is in excess of $6.9 billion; including medical costs, missed work time and the costs of premature deaths. With the US food and agricultural industries totaling in excess of $306 billion in revenues annually, the amount spent on monitoring safety, quality and preventing foodborne illness is staggering. Foodborne diseases are caused by ingesting bacteria, fungi, parasites, viruses or their toxins through contaminated food or drinking water. More than 200 known diseases are transmitted through food, and more than half of all recognized foodborne disease outbreaks have unknown causes, indicating the real number of disease-causing agents is likely much larger than 200. The symptoms of foodborne illnesses range from mild gastroenteritis to life-threatening neurological, hepatic, and renal syndromes. A pathogen's ability to cause illness can be very different from the severity of the illness it causes. Some pathogens cause a great number of illnesses but the severity is low. Others cause few illnesses, but those illnesses are very severe or even fatal. In addition to disease caused by direct infection, some foodborne diseases are caused by the presence of a toxin in the food that was produced by a microbe in the food. Staphylococcus aureus can grow in food and produce a toxin that causes severe vomiting. The rare yet deadly disease botulism occurs when Clostridium botulinum grows in food and produces a powerful paralytic toxin. These toxins can produce illness even if the microbes that produced them are no longer there, commonly having been killed by some heat treatment as with canned foods. Foodborne pathogens can cause actual infections in the bloodstream or tissues, in the gut or simply pass thru leaving toxins behind which cause the symptoms of foodborne illness. Each agent has a specific typical progression of illness and symptoms. Since symptoms commonly include vomiting and diarrhea, a laboratory test is required to identify the causative agent. The major culprits in bacterial foodborne illness are Salmonella, Listeria, Campylobacter, Clostridium, Shigella, E. coli 0157:H7 and Staphylococcus. Listeria and E. coli 0157:H7 are also considered emerging pathogens since there role in food illness have only become well recognized in the past 20 years. The first major outbreak of E. coli 0157:H7 was reported in 1982 and an early major outbreak with Listeria occurring in 1985 from California produced soft cheese. Recent outbreaks in fresh produce of E. coli 0157:H7 from a number of sources demonstrate that this previously animal product (beef) associated pathogen is a threat throughout the food system. Another important and common emerging pathogen is not a bacterium at all, but a virus. The Calicivirus or Norwalk-like virus is an extremely common causative agent but is rarely diagnosed due to a lack of a widely available laboratory test for it. It causes an acute gastrointestinal illness, usually with more vomiting than diarrhea, which resolves within two days. Unlike many foodborne pathogens that have animal reservoirs, it is believed that Norwalk-like viruses spread primarily from one infected person to another. The frequency of outbreaks caused by these emerging organisms has been increasing worldwide, including in the western developed countries. New foodborne disease threats occur for a number of reasons. These include increase in international travel and trade, microbial adaptation, changes in the food production system, and human demographics or behavior pattern shifts. Food Safety Information Links: The Gateway to US Government Food Safety Information http://www.foodsafety.gov/ The US Food & Drug Administration http://www.cfsan.fda.gov/list.html USDA-FSIS Food Safety http://www.fsis.usda.gov/Fact_Sheets/ Foodborne_Illness_&_Disease_Fact_Sheets/index.asp CDC Foodborne Infectious Agents http://www.cdc.gov/ncidod/dbmd/diseaseinfo/ foodborneinfections_g.htm A Reference Guide for Foodborne Pathogens http://edis.ifas.ufl.edu/FS127 Foodborne Illness Basics http://www.health.state.mn.us/divs/idepc/ dtopics/foodborne/basics.html When is Culture Based Analysis Warranted?Dr. Florence Wu, Principal Mycologist, Aemtek, Inc. Using spore-trapping samplers to collect fungal spores and then direct microscopy to identify and enumerate the spores is a common method in indoor air quality (IAQ) surveys. This practice has its practical merits, but it has limitations. One problem is that the direct examination of the samples often results in uncertainty in the identification of the fungi involved. Only a small number of fungal spore types can be identified to generic level with confidence. Species identification solely based on spore morphology is usually unachievable. Even the most significant genera such as Aspergillus and Penicillium cannot be confirmatively differentiated on spore -trap samples. This problem hinders the accurate assessment of fungal contaminants in IAQ projects. Culture based methods offer a convenient and practical solution to this problem, but generally require a seven-day incubation period. Culture based methods use artificial media to isolate microorganisms from environmental samples for the purpose of identification and enumeration. The fungi that are capable of growing on indoor building materials (mostly dead organic materials) are primarily saprophytic, which means they can usually be cultured on artificial media. Almost all common indoor fungi can be grown on media and produce sporulating colonies within a reasonable time frame (about 5-14 days). In addition, the spores of many indoor fungal species can remain viable and culturable for a very long time (e.g., years) which makes recovery a relatively easy task. Culture based analytical methods take advantage of these facts and allow for more detailed analysis. Culture-based methods can be efficiently used to assess indoor fungi quantitatively and at species level. Air, surface swab, liquid, bulk, and dust samples are suitable for culture based analysis, but tape lift samples are not appropriate for culturing in most cases. There are of course many factors influencing the results of culture based analysis. This article, however, concentrates on the situations when culture based analysis is called for in IAQ investigations. Species Identification The study and naming of filamentous fungi (including molds) mostly rely on the morphological characteristics of the colony, hyphae, sporulating mechanisms and structures, and spores. These characteristics are best observed through obtaining pure cultures of the organisms. Except for molecular methods, obtaining cultures of filamentous fungi is essential for identifying the fungi to species. Most filamentous species are discovered, described, and referenced based on the full body of the representative culture (called “type specimen”). Identification is achieved by comparing the unknown organism with the type specimen or the original/reliable description of the type specimen. This approach is time-honored and scientifically sound. In IAQ surveys where species identification is necessary, a culture-based method can be readily employed to identify the fungal contaminants. The term “species identification” should not be confused with “speciation”. These two terms are not interchangeable. Species identification refers to a human activity of finding out the identity of an organism to species that has been discovered, described, and given a scientific name. “Speciation” refers to the forming of new species and it is an evolutionary process consisting of gene mutation, genetic exchange, geographical isolation, natural selection, etc. Confirmative Identification of Contamination Source Most IAQ specialists would agree that if the results of spore trap samples indicated significantly elevated airborne spore concentration, the source of the indoor fungal contamination or growth needs to be located. Direct examination alone can generate assumptive identification but normally does not lead to confirmative identification. For example, there was an Aspergillus species with small, spherical and smooth spores (e.g., A. versicolor) growing on the wall surface. The Aspergillus/Penicillium-like spores in the spore trap sample could be Aspergillus or Penicillium species with small, spherical, and smooth spores. How can you be sure that the Aspergillus growth you observed on the wall is responsible for the elevated airborne Aspergillus/Penicillium-like spore level, not any other fungal growth hidden somewhere? Only culture or molecular based methods can confirmatively coordinate the growth on the wall is the same species captured on the spore trap sample. Pathogenic Fungi Because only live pathogens can cause disease and the viability of a microorganism determines its infectivity and growth potential, the use of culturable methods is required in certain environments, such as in public buildings or in the critical areas of hospitals and in clean rooms. Some other IAQ investigations may also target at fungi that post potential health risk to the building residents. Culture based method is the only way to provide data on culturable fungi from nearly all sample types (spore trap sample with unsolvable adhesives is an exception). The sampling and analytical protocols can be designed to target specific groups, for example, human pathogens. Initial and Post-remediation Testing One of the reasons that culture-based method is not as widely used as direct examination, despite its important benefits, is the waiting period necessary for fungal incubation. QPCR has the advantage for being fast, sensitive, and species level identification, but if the list of species to be detected is too long, and then it will be too costly. One possible solution is using the culture based method in the initial investigation to isolate and identify the fungal growth that needs to be removed. While the remediation is in progress, the contaminate is identified to species level, and such a specifically targeted QPCR panel can be designed. This will allow the use of the QPCR method to rapidly verify the adequate cleaning of the problem organism(s) in a highly, sensitive and legally defensible fashion. Cost Effectiveness Culture based analysis is one of the most economical ways to identify molds to species level, especially when compared to existing molecular methods. Specific targets have to be defined before a real time PCR can be run. For initial investigation, using molecular method to screen unknown species is probably cost-inhibitive in most cases. In addition, molecular method alone cannot assess the viability of the organisms. Due to these reasons, culture based methods are still the most commonly used methods to and to provide valuable information that cannot be obtained otherwise. Comments? E-mail florencewu@aemtek.com Aemtek's Features and Services Aemtek Analytical Services Mold – spore trap, bulk, tape-lift, swab, dust Culturable fungi Environmental bacteria Legionella pneumophila Sewage contamination screen Allergens Microbial characterization in special environments Microbiology/mycology research, consultation, and legal support Material microbial resistence testing Service Features Report via e-mail or fax. Online report access 24/7 through our secure client login area. Report supporting documents are available online. Microbial Database posted on http://www.aemtek.com/ Free subscription of Aemtek newsletter, The Micro Examiner Accurate Data We operate with vigorous quality assurance and quality control. Our analysts have advanced degrees and years of experience. Aemtek is AIHA EMLAP accreditated and California ELAP certified. Reliable Service Consistency in data quality, integrity, and excellent service. Responsive and courteous customer service. On time reporting. 100% customer satisfaction guarantee. Extra Value to Clients Free sampling supplies, including swabs, media, and tape-lift kits. Free sample shipping (for projects exceeding $200). Free phone consultation with our principal analyst. Fast and Affordable Highly competitive price schedule. Expedited service at a reasonable price. Weekend analysis available.
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